Plasmid

Part:BBa_K4129033

Designed by: Giovanni Carlo Gendarini   Group: iGEM22_DTU-Denmark   (2022-10-11)


Transient expression system for BBa_K4129004

Plasmid to transiently express BBa_K4129004 in Aspergillus niger.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5340
    Illegal EcoRI site found at 7617
    Illegal EcoRI site found at 9442
    Illegal XbaI site found at 3519
    Illegal XbaI site found at 4285
    Illegal XbaI site found at 4767
    Illegal XbaI site found at 5535
    Illegal XbaI site found at 7422
    Illegal XbaI site found at 8190
    Illegal XbaI site found at 8672
    Illegal SpeI site found at 3702
    Illegal SpeI site found at 3713
    Illegal SpeI site found at 4077
    Illegal SpeI site found at 4209
    Illegal SpeI site found at 4333
    Illegal SpeI site found at 4440
    Illegal SpeI site found at 4451
    Illegal PstI site found at 6484
    Illegal PstI site found at 10280
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5340
    Illegal EcoRI site found at 7617
    Illegal EcoRI site found at 9442
    Illegal NheI site found at 10936
    Illegal SpeI site found at 3702
    Illegal SpeI site found at 3713
    Illegal SpeI site found at 4077
    Illegal SpeI site found at 4209
    Illegal SpeI site found at 4333
    Illegal SpeI site found at 4440
    Illegal SpeI site found at 4451
    Illegal PstI site found at 6484
    Illegal PstI site found at 10280
    Illegal NotI site found at 3558
    Illegal NotI site found at 10927
    Illegal NotI site found at 10981
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5340
    Illegal EcoRI site found at 7617
    Illegal EcoRI site found at 9442
    Illegal BglII site found at 1915
    Illegal BglII site found at 9344
    Illegal BamHI site found at 2175
    Illegal BamHI site found at 9482
    Illegal BamHI site found at 10018
    Illegal XhoI site found at 278
    Illegal XhoI site found at 519
    Illegal XhoI site found at 5881
    Illegal XhoI site found at 6598
    Illegal XhoI site found at 7076
    Illegal XhoI site found at 9878
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5340
    Illegal EcoRI site found at 7617
    Illegal EcoRI site found at 9442
    Illegal XbaI site found at 3519
    Illegal XbaI site found at 4285
    Illegal XbaI site found at 4767
    Illegal XbaI site found at 5535
    Illegal XbaI site found at 7422
    Illegal XbaI site found at 8190
    Illegal XbaI site found at 8672
    Illegal SpeI site found at 3702
    Illegal SpeI site found at 3713
    Illegal SpeI site found at 4077
    Illegal SpeI site found at 4209
    Illegal SpeI site found at 4333
    Illegal SpeI site found at 4440
    Illegal SpeI site found at 4451
    Illegal PstI site found at 6484
    Illegal PstI site found at 10280
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5340
    Illegal EcoRI site found at 7617
    Illegal EcoRI site found at 9442
    Illegal XbaI site found at 3519
    Illegal XbaI site found at 4285
    Illegal XbaI site found at 4767
    Illegal XbaI site found at 5535
    Illegal XbaI site found at 7422
    Illegal XbaI site found at 8190
    Illegal XbaI site found at 8672
    Illegal SpeI site found at 3702
    Illegal SpeI site found at 3713
    Illegal SpeI site found at 4077
    Illegal SpeI site found at 4209
    Illegal SpeI site found at 4333
    Illegal SpeI site found at 4440
    Illegal SpeI site found at 4451
    Illegal PstI site found at 6484
    Illegal PstI site found at 10280
    Illegal NgoMIV site found at 6618
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9211
    Illegal BsaI site found at 10071
    Illegal BsaI.rc site found at 193
    Illegal BsaI.rc site found at 345
    Illegal BsaI.rc site found at 3746


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Categories
Parameters
None