RNA

Part:BBa_K4127020

Designed by: Xiongbing Yang   Group: iGEM22_CSU_CHINA   (2022-10-09)

mgs14 sgRNA

We designed sgRNA to guide the CRISPR Cas12a protein and activate trans-cleavage activity. The sgRNA squence based on the cell-free DNA sequence of Echinococcosis. The target sequence of this sgRNA is mgs14.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source:

The target sequences of sgRNA were derived from Echinococcus cell-free DNA.

Experiment:

1.CRISPR Cas12a system for fluorescence detection:

CRISPR Cas12a system for fluorescence detection

Result:

Figure 1:The sgRNA can activate the CRISPR Cas12a protein in trans-cleavage activity.
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Categories
Parameters
None