Primer

Part:BBa_K4127013

Designed by: Xiongbing Yang   Group: iGEM22_CSU_CHINA   (2022-10-09)

mgs14 F

We designed primers based on the cell-free DNA sequence of Echinococcosis. The amplicon sequence of this primer pair was mgs14.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source:

The amplicon sequences of primer pairs were derived from Echinococcus cell-free DNA.

Experiment:

1.RPA system:

RPA system

2.Purify the RPA product:


RPA product: trichloromethane: phenol =2:1:1
Refer to the above system to add 15 μL RPA product, 7.5 μL trichloromethane and 7.5 μL phenol,then mix and centrifuge at 13,000 rpm for 10 min.

Result:

Figure 1:There is a clear band near the expected length, and the sequence result is consistent with the expected sequence.
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Parameters
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