Primer
Part:BBa_K4127013
Designed by: Xiongbing Yang Group: iGEM22_CSU_CHINA (2022-10-09)
mgs14 F
We designed primers based on the cell-free DNA sequence of Echinococcosis. The amplicon sequence of this primer pair was mgs14.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source:
The amplicon sequences of primer pairs were derived from Echinococcus cell-free DNA.
Experiment:
1.RPA system:
2.Purify the RPA product:
RPA product: trichloromethane: phenol =2:1:1
Refer to the above system to add 15 μL RPA product, 7.5 μL trichloromethane and 7.5 μL phenol,then mix and centrifuge at 13,000 rpm for 10 min.
Result:
[edit]
Categories
Parameters
None |