Part:BBa_K4127003

mgs4 F

We designed primers based on the cell-free DNA sequence of Echinococcosis. The amplicon sequence of this primer pair was mgs4.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Source:

The amplicon sequences of primer pairs were derived from Echinococcus cell-free DNA.

1.RPA system:

RPA system

2.Purify the RPA product:

RPA product: trichloromethane: phenol =2:1:1
Refer to the above system to add 15 μL RPA product, 7.5 μL trichloromethane and 7.5 μL phenol,then mix and centrifuge at 13,000 rpm for 10 min.

Result:

Figure 1:There is a clear band near the expected length, and the sequence result is consistent with the expected sequence.