Composite

Part:BBa_K4123001

Designed by: Franco Herrera   Group: iGEM22_Manchester   (2022-10-11)


Plasmid for the synthesis of Auxin (IAA)

The plasmid uses pBbE8c-RFP as vector, the AldH, KDC, and ARO8 genes are inserted. ARO8 is a aminotransferase that converts L-tryptophan to indole-3-pyruvic acid. KDC is a decarboxylase that mediates the decarboxylation of indole-3-pyruvic acid to indole-3-acetaldehyde. Finally. AldH is a aldehyde dehydrogenase, which function is the oxidation of indole-3-acetaldehyde to the corresponding IAA.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 501
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1305
    Illegal EcoRI site found at 1623
    Illegal EcoRI site found at 3466
    Illegal EcoRI site found at 3627
    Illegal XbaI site found at 16
    Illegal XbaI site found at 1638
    Illegal XbaI site found at 3642
    Illegal SpeI site found at 3599
    Illegal SpeI site found at 5184
    Illegal PstI site found at 1438
    Illegal PstI site found at 3613
    Illegal PstI site found at 5198
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1305
    Illegal EcoRI site found at 1623
    Illegal EcoRI site found at 3466
    Illegal EcoRI site found at 3627
    Illegal NheI site found at 1290
    Illegal SpeI site found at 502
    Illegal SpeI site found at 3599
    Illegal SpeI site found at 5184
    Illegal PstI site found at 516
    Illegal PstI site found at 1438
    Illegal PstI site found at 3613
    Illegal PstI site found at 5198
    Illegal NotI site found at 7
    Illegal NotI site found at 509
    Illegal NotI site found at 1629
    Illegal NotI site found at 3606
    Illegal NotI site found at 3633
    Illegal NotI site found at 5191
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1305
    Illegal EcoRI site found at 1623
    Illegal EcoRI site found at 3466
    Illegal EcoRI site found at 3627
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 502
    Illegal EcoRI site found at 1305
    Illegal EcoRI site found at 1623
    Illegal EcoRI site found at 3466
    Illegal EcoRI site found at 3627
    Illegal XbaI site found at 1638
    Illegal XbaI site found at 3642
    Illegal SpeI site found at 3599
    Illegal SpeI site found at 5184
    Illegal PstI site found at 1438
    Illegal PstI site found at 3613
    Illegal PstI site found at 5198
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 1305
    Illegal EcoRI site found at 1623
    Illegal EcoRI site found at 3466
    Illegal EcoRI site found at 3627
    Illegal XbaI site found at 16
    Illegal XbaI site found at 1638
    Illegal XbaI site found at 3642
    Illegal SpeI site found at 502
    Illegal SpeI site found at 3599
    Illegal SpeI site found at 5184
    Illegal PstI site found at 516
    Illegal PstI site found at 1438
    Illegal PstI site found at 3613
    Illegal PstI site found at 5198
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 27
    Illegal BsaI site found at 1649
    Illegal BsaI site found at 3652
    Illegal BsaI.rc site found at 1603
    Illegal BsaI.rc site found at 3586
    Illegal BsaI.rc site found at 5171
    Illegal SapI site found at 574


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