Composite

Part:BBa_K4121086

Designed by: Ziyu Li   Group: iGEM22_NEFU_China   (2022-10-05)


Expression cassette of IDI with constitutive promoter

The part is called "pCCW12-IDI-tPRM9". We use pCCW12 as the promoter, tPRM9 as the terminator and the CDS of this part is IDI. The three basic parts above make up a new transcription unit, Isopentenyl pyrophosphate isomerase is a key rate-limiting enzyme in terpene biosynthesis, catalyzing the interconversion between IPP and dimethylallyl diphosphate (DMAPP) in mevalonate pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 240
    Illegal NheI site found at 1431
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 240
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 240
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None