Composite

Part:BBa_K4121082

Designed by: Ziyu Li   Group: iGEM22_NEFU_China   (2022-10-05)


Expression cassette of ERG10 with constitutive promoter

The part is called "pCCW12-ERG10-tCPS1". We use pCCW12 as the promoter, tCPS1 as the terminator and the CDS of this part is ERG10. The three basic parts above make up a new transcription unit, It has the function to catalyze the conversion of Acetyl-CoA to Acetoacetyl-CoA in mevalonate pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 240
    Illegal EcoRI site found at 1465
    Illegal PstI site found at 1280
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 240
    Illegal EcoRI site found at 1465
    Illegal PstI site found at 1280
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 240
    Illegal EcoRI site found at 1465
    Illegal BglII site found at 1204
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 240
    Illegal EcoRI site found at 1465
    Illegal PstI site found at 1280
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 240
    Illegal EcoRI site found at 1465
    Illegal PstI site found at 1280
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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