Part:BBa_K4121081
Expression cassette of ERG8 with constitutive promoter
The part is called "pPGL1-ERG8-tCPS1". We use pCCW12 as the promoter, tCPS1 as the terminator and the CDS of this part is ERG8. The three basic parts above make up a new transcription unit, it has the function to catalyze the conversion of mevalonate-P to mevalonate-PP in mevalonate pathway.
Design Notes
We used the "Goldengate" method for the ligation of the basic parts, We introduced recognition sites and cleavage sites for the type IIs restriction endonuclease BsmBI at both ends of the Basic Parts to ensure that the Basic Parts could be ligated successfully, and changed the location of the recognition sites and cleavage sites so that the BsmBI would not cut repeatedly. To avoid homologous recombination in Saccharomyces cerevisiae, we substitute different promoters and terminators. Please refer to" Design" part of the Wiki for detailed experimental design.
Source
We get sequences of the promoter from Eukaryotic promoter database(EPD), the sequences of CDS and the terminator from National Center for Biotechnology Information(NCBI).
References
None
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1426
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1426
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1426
- 1000COMPATIBLE WITH RFC[1000]
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