DNA

Part:BBa_K4121034

Designed by: Ziyu Li   Group: iGEM22_NEFU_China   (2022-10-05)


single-copy homologous arms downstream sequence on the Saccharomyces cerevisiae genome

Homology arm usually refers to the flanking sequence on both sides of the exogenous sequence to be inserted in the target vector, which is completely consistent with the genome sequence, and is used to identify and recombine the regions. Our team found a sequence in the loose region of the Saccharomyces cerevisiae genome that serves as an downstream homology arm to integrate our target gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 368


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Categories
Parameters
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