DNA
Part:BBa_K4121034
Designed by: Ziyu Li Group: iGEM22_NEFU_China (2022-10-05)
single-copy homologous arms downstream sequence on the Saccharomyces cerevisiae genome
Homology arm usually refers to the flanking sequence on both sides of the exogenous sequence to be inserted in the target vector, which is completely consistent with the genome sequence, and is used to identify and recombine the regions. Our team found a sequence in the loose region of the Saccharomyces cerevisiae genome that serves as an downstream homology arm to integrate our target gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 368
[edit]
Categories
Parameters
None |