DNA

Part:BBa_K4121008

Designed by: Ziyu Li   Group: iGEM22_NEFU_China   (2022-10-04)


It is a promoter in Saccharomyces cerevisiae, the expression intensity is relatively strong.

pCCW12 promoter can initiate the expression of CCW12 gene in Saccharomyces cerevisiae. Through our characterization of mCherry as an example, the promoter has strong promoter activity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 240
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 240
  • 1000
    COMPATIBLE WITH RFC[1000]


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