Coding

Part:BBa_K4115001

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-19)


superfolder GFP with LVA

This part is the coding sequence of superfolder green fluorescence protein(sfGFP). An LVA degradation tag is added to its C terminus to decrease its half-life. Its maximum excitation wavelength is 485nm. And its maximum emission wavelength is 510nm. sfGFP can fold correctly even when fused to some poorly folded proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None