Coding

Part:BBa_K4113010

Designed by: Wenhui Ren   Group: iGEM22_FAFU-China   (2022-10-06)


DlGAD5

Glutamic acid decarboxylase (GAD) is a key rate-limiting enzyme in the synthesis pathway of the inhibitory neurotransmitter γ-aminobutyric acid (GABA), which plays an important function in plant cellular adversity defense, nitrogen storage, hormone synthesis, and growth and development. When plants are subjected to external environmental stimuli (e.g. mechanical damage, drought, frost, etc.), intracellular H+ or Ca2+ levels increase, which in turn leads to the activation of GAD and a significant increase in GABA content

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 774
    Illegal PstI site found at 350
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 402
    Illegal PstI site found at 350
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 774
    Illegal PstI site found at 350
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 774
    Illegal PstI site found at 350
    Illegal AgeI site found at 293
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None