Composite

Part:BBa_K4103023

Designed by: Almiro Pires da Silva Neto   Group: iGEM21_Open_Science_Global   (2021-10-20)


Secretion Tag library 2

Library plasmid containing Bacillus subtilis secretion tags and associated ribosome binding sites, defined as RBSLoc parts in the ProClo/expanded FreeGenes MoClo assembly standard. Digestion with BsaI will free all the parts to participate in a Moclo assembly reaction, the transformants of which can then be screened for secretion efficiency. PmeI could be added to the digestion reaction to prevent multiple tags from assembling in tandem into the finished constructs. Plasmid 1 of 6 in the library.

Available at https://stanford.freegenes.org/products/b-subtilis-protein-secretion-toolkit?_pos=1&_psq=se&_ss=e&_v=1.0#description

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2528
    Illegal PstI site found at 4483
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2528
    Illegal PstI site found at 4483
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2528
    Illegal PstI site found at 4483
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2528
    Illegal PstI site found at 4483
    Illegal AgeI site found at 3213
    Illegal AgeI site found at 4010
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 195
    Illegal BsaI site found at 383
    Illegal BsaI site found at 583
    Illegal BsaI site found at 795
    Illegal BsaI site found at 1020
    Illegal BsaI site found at 1214
    Illegal BsaI.rc site found at 121
    Illegal BsaI.rc site found at 309
    Illegal BsaI.rc site found at 509
    Illegal BsaI.rc site found at 721
    Illegal BsaI.rc site found at 945
    Illegal BsaI.rc site found at 1140
    Illegal BsaI.rc site found at 1337


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