Plasmid

Part:BBa_K4103010

Designed by: Almiro Pires da Silva Neto   Group: iGEM21_Open_Science_Global   (2021-10-01)


pOpenv3

Robust, flexible E. coli cloning vector. Designed to be compatible with multiple cloning strategies including restriction ligation, Gibson assembly, SLiCE, and Golden Gate assembly using the FreeGenes MoClo standard.

Available at https://stanford.freegenes.org/collections/open-genes/products/open-plasmids#genes

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2089


Name: 8a-1a: Pfu-Sso7d_noCtag | Codon Optimized By Strategy #2 Bacillus Subtilis Starvation Genes BsaI Prefix: AATG BsaI suffix: AGGT

Original Pfu-sso7d sequence from https://github.com/Koeng101/pichia_toolkit -> https://patents.google.com/patent/US6627424B1/en

AA 1-844 exactly matches the patent sequence.

Codon Amino Acid Identity based on: https://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi#SG11

Optimized codons with own software based on generated codon tables from select genome: https://www.embopress.org/doi/full/10.15252/msb.20156236

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