Part:BBa_K4103004
EcoOri
High copy origin of replication.
Biology
Origins of replication are basic plasmid elements where the DNA replication begins, this replication enable the plasmid to reproduce itself and survive within cells[1].
The colE1 is a origin of replication found in E.coli that is widely used in some common vectors: pMB1, pBR322, pUC. The ColE1 fragment contains two promoters, one of them is responsible for the initiation of the replication primer RNA (RNA II) and the other promoter (RNA I) forms a tightle folded RNA that is complementary to the nascent RNA II.
Usage
The ColE1 origin of replication was used for replication and mantainance of our plasmid in E. coli strain before final conjugation with our chassis B. Subtilis.
Name: EcoOri ColE1pMB1pBR322pUC BsaI Prefix: ACTA BsaI suffix: AAAA
From pHT43 - https://www.mobitec.com/media/datasheets/mobitecgmbh/Bacillus_subtilis_pgrac01_expression_vectors-handbook.pdf Sequence from NCBI Blast
This E. coli origin of replication is widely used in some common vectors: pMB1, pBR322, pUC.
NCBI BLAST of entire sequence: Conclusion: BLAST confirms that the part sequence is good!
References
[1]Morgan K. Plasmids 101: Origin of Replication. Addgene.org. Published 2020. Accessed October 20, 2021. https://blog.addgene.org/plasmid-101-origin-of-replication
[2]Cesareni G, Helmer-Citterich M, Castagnoli L. Control of ColE1 plasmid replication by antisense RNA. Trends in Genetics. 1991;7(7):230-235. doi:10.1016/0168-9525(91)90370-6
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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