Composite

Part:BBa_K4103002

Designed by: Almiro Pires da Silva Neto   Group: iGEM21_Open_Science_Global   (2021-10-13)


kanR

KanR selection - promoter + coding region + terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Name: Ecoli_KanR BsaI Prefix: GCAA BsaI suffix: ACTA

Kanamycin selection marker for E.coli. From: SEVA cloning vectors [i.e. pSEVA233 - https://www.ncbi.nlm.nih.gov/nuccore/JX560356]

CDS 830..1645 gene="neo" codon_start=1 transl_table=11 product="neomycin phosphotransferase" protein_id="AFV59842.1" translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"

[edit]
Categories
Parameters
None