Coding

Part:BBa_K4102000

Designed by: Friedrich J. Ehinger   Group: iGEM21_Bonn-Rheinbach   (2021-10-07)


lanM gene

This 363 bp sequences encodes the bacterial protein lanmodulin (LanM), including an N-terminally added hexa-His-tag. LanM can selectively chelate rare earth element (REE) cations at high affinity even for picomolar concentrations. This is possible due to four EF-hand motifs. The naturally present translocation tag was not included.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 109
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None