Coding

Part:BBa_K4096001

Designed by: Ke ZHU   Group: iGEM21_Shanghai_Metro_HS   (2021-10-12)

PKC-001


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 25
    Illegal XhoI site found at 97
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 133
    Illegal NgoMIV site found at 331
    Illegal NgoMIV site found at 688
    Illegal NgoMIV site found at 1025
  • 1000
    COMPATIBLE WITH RFC[1000]

BBa_K4096001

Name: PKC-001

Base Pairs: 1077bp

Origin: Pseudomonas aeruginosa, genome

Properties: A coding sequence of alkali cellulase.

Usage and Biology

BBa_K4096001 is a coding sequence of alkali cellulase (PKC-001) from Pseudomonas aeruginosa. Cellulases break down the cellulose molecule into monosaccharides ("simple sugars") such as beta-glucose, or shorter polysaccharides and oligosaccharides. Several different kinds of cellulases are known, which differ structurally and mechanistically. Synonyms, derivatives, and specific enzymes associated with the name "cellulase" include endo-1,4-beta-D-glucanase (beta-1,4-glucanase, beta-1,4-endoglucan hydrolase, endoglucanase D, 1,4-(1,3,1,4)-beta-D-glucan 4-glucanohydrolase), carboxymethyl cellulase (CMCase), avicelase, celludextrinase, cellulase A, cellulosin AP, alkali cellulase, cellulase A 3, 9.5 cellulase, and pancellase SS.

References

MUCK R,NADEAU E,MCALLISTER T,et al. Silage review: recent advances and future uses of silage additives [J]. J Dairy Sci.

刘海燕,张鹏举,王秀飞,等. 正交试验法优化玉米秸秆穰叶青贮发酵剂的研究 [J]. 中国饲料,2018 ( 11) : 74-79.

Coward-Kelly G., Aiello-Mazzari C., Kim S., Granda C., and Holtzapple M., 2003, Suggested improvements to the standard filter paper assay used to measure cellulase activity,Biotechnology & Bioengineering, 82(6): 745-749.

Ghose T.K., 1987, Measurement of cellulase activities, Pure & Appl Chem, 59(2): 257-268.

Lu Z.L., Chen D., Zhang S.S., Lu Q., and Huang R.B., 2012, A Pseudomonas aeruginosa producing alkaline cellulase, China Patent, 2012103821716

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