Part:BBa_K4092021

Toehold for detection of RSV A

Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C for the detection of Respiratory Syncytial Virus (RSV) type A, has a MFE of -18 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.

Usage and Biology

In order to have a more graphical approach of the comparison of the structures, we obtained an image of the secondary structure of the toeholds using the NUPACK package.

Fig. Toehold secondary structure at 37°C.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]