Part:BBa_K4092014
Specificity control toehold for Agave tequilana
Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C and works as a negative control for the detection of Agave Tequilana (SST1 gene), since it has a set of mutations every 8 bp on the SST1 gene complementary region it should not correctly interact with the toehold and thus not express any reporter gene. It was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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