Generator

Part:BBa_K408000

Designed by: Pavan Choppakatla   Group: iGEM10_IIT_Delhi_1   (2010-11-05)


GFP with signal peptide for hemolysin system

This GFP has been fused with the signal sequence for the alpha-hemolysin system to enable this peptide to be secreted by all E.coli and even other species with the generic trans membrane protein complex made by proteins coded by genes hlyB and hlyD.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
    Illegal SpeI site found at 953
    Illegal PstI site found at 835
    Illegal PstI site found at 868
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 953
    Illegal PstI site found at 835
    Illegal PstI site found at 868
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
    Illegal SpeI site found at 953
    Illegal PstI site found at 835
    Illegal PstI site found at 868
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
    Illegal SpeI site found at 953
    Illegal PstI site found at 835
    Illegal PstI site found at 868
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 670


[edit]
Categories
Parameters
n/aGFP with signal peptide for hemolysin system