Tag

Part:BBa_K4077010

Designed by: Himanshu Ranjan   Group: iGEM21_NIT_Warangal   (2021-10-20)


PpHIS4

Pichia wild-type gene coding for histidinol dehydrogenase (~2.5 kb) and used to complement Pichia his4 strains.

Usage and Biology

Provides a selectable marker to isolate Pichia recombinant strains.

References

(User Manual PPIC9K a Pichia Vector for Multicopy Integration and Secreted Expression Catalog No. V175-20)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 53
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 761
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 53
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2101


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