Part:BBa_K4077005

Alpha-factor secretion signal

Native Saccharomyces cerevisiae alpha-factor secretion signal that allows for efficient secretion of most proteins from Pichia.

Usage and Biology

The yeast, Pichia pastoris, has been genetically engineered to produce many heterologous proteins for industrial and research purposes. In order to secrete proteins for easier purification from the extracellular medium, the coding sequence of recombinant proteins are initially fused to the Saccharomyces cerevisiae α-mating factor secretion signal leader. Addition of the α-mating factor secretion signal to heterologous proteins in Pichia pastoris usually results in the secretion of the intact heterologous protein to the extracellular medium. [1]

References

[1]Lin-Cereghino, G.P., Stark, C.M., Kim, D., Chang, J., Shaheen, N., Poerwanto, H., Agari, K., Moua, P., Low, L.K., Tran, N., Huang, A.D., Nattestad, M., Oshiro, K.T., Chang, J.W., Chavan, A., Tsai, J.W. and Lin-Cereghino, J. (2013). The effect of α-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris. Gene, [online] 519(2), pp.311–317. Available at: http://www.sciencedirect.com/science/article/pii/S0378111913001765.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 23
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 23
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 244
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 23
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 23
  
• 1000
  COMPATIBLE WITH RFC[1000]