Composite

Part:BBa_K4062034

Designed by: Benjamin Bateman   Group: iGEM21_WLC-Milwaukee   (2021-10-21)


lacI with lac operon regulatory components, lacO mutation

This part includes a mutated lac operator. The fifth and sixth bases of lacO were changed from GT to AC. This part also contains the lacI promoter, the lacI ORF, CAP site, lacP, and lacZ RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None