Composite

Part:BBa_K4062033

Designed by: Benjamin Bateman   Group: iGEM21_WLC-Milwaukee   (2021-10-21)


lacI with lac operon regulatory components, lacI ORF mutation

This part includes a mutated lacI open reading frame. The third and fourth codons in the lacI ORF are changed to stop codons. The part also includes the lacI promoter, CAP binding site, lacP, lacO, and the lacZ RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None