Composite

Part:BBa_K4062030

Designed by: Benjamin Bateman   Group: iGEM21_WLC-Milwaukee   (2021-10-21)


lacI with lac operon regulatory components, lacI promoter deletion

This composite part includes a mutated promoter for lacI. Six total bases were removed from the -10 region.

This part also contains the lacI ORF, CAP site, lacP, lacO, and lacZ RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None