Composite

Part:BBa_K4062015

Designed by: Benjamin Bateman   Group: iGEM21_WLC-Milwaukee   (2021-10-15)


lacY ORF and RBS

This part includes the lacY open reading frame and its ribosome binding site. It can be paired with Bba_K4062014 to regulate expression using environmental lactose similar to Bba_K4062016. Additionally, this part can be used when uptake of lactose or another galactoside from the environment is necessary.

T--WLC-Milwaukee--lacYgel.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 787
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None