Composite

Part:BBa_K4062014

Designed by: Benjamin Bateman   Group: iGEM21_WLC-Milwaukee   (2021-10-15)


lacI gene with lac operon regulatory components

This composite part includes the promoter and open reading frame of lacI with the regulatory components of the lac operon downstream. Additionally, there is a promoter and ribosome binding site for any gene inserted downstream of Bba_K4062014, allowing for lactose regulated expression. This part can be paired with Bba_K4062015 to ensure lactose or other galactoside's integration into the cell and subsequent regulation.

This part is included in Bba_K4062016 which demonstrates its function using bla.

T--WLC-Milwaukee--lacIgel2.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None