Regulatory

Part:BBa_K4062011

Designed by: Benjamin Bateman   Group: iGEM21_WLC-Milwaukee   (2021-10-15)


CAP binding site, inactivated by deletion

The CAP site of the Escherichia coli lac operon. The first five bases are removed. This mutation reduces the binding of the CAP protein to CAP site [1].


[1]: Xian-Ming Yu and William S. Reznikoff. "Deletion analysis of the CAP-cAMP binding site of the Escherichia coli lactose promoter." Nucleic Acids Research 12:13. 1984.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None