Part:BBa_K4060520

BBa_K4060513 + BBa_K4060517 + BBa_K4060504

The part that establishes the expression of BphP1, QPAS1, and HO1, and also contains a GFP reporter gene (BBa_K763004) that report the LexA signal in the cell. This part is for functional test of BphP1-LexA_QPAS1 optogenetic system. If the optogenetic system functions properly, the production of GFP should increase while under NIR.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3064
  Illegal SpeI site found at 3100
  Illegal PstI site found at 963
  Illegal PstI site found at 1260
  Illegal PstI site found at 3457
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3064
  Illegal NheI site found at 144
  Illegal NheI site found at 167
  Illegal NheI site found at 2551
  Illegal NheI site found at 2574
  Illegal NheI site found at 3965
  Illegal NheI site found at 3988
  Illegal SpeI site found at 3100
  Illegal PstI site found at 963
  Illegal PstI site found at 1260
  Illegal PstI site found at 3457
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3064
  Illegal BamHI site found at 619
  Illegal XhoI site found at 2369
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3064
  Illegal SpeI site found at 3100
  Illegal PstI site found at 963
  Illegal PstI site found at 1260
  Illegal PstI site found at 3457
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3064
  Illegal SpeI site found at 3100
  Illegal PstI site found at 963
  Illegal PstI site found at 1260
  Illegal PstI site found at 3457
  Illegal NgoMIV site found at 282
  Illegal NgoMIV site found at 570
  Illegal NgoMIV site found at 605
  Illegal NgoMIV site found at 1853
  Illegal NgoMIV site found at 2355
  Illegal NgoMIV site found at 2761
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1410
  Illegal BsaI.rc site found at 2256