Composite

Part:BBa_K4060518

Designed by: Cheng-Ju Lu   Group: iGEM21_NYCU-Taipei   (2021-10-21)


BBa_K4060503 + BBa_K4060509

mCherry_QPAS1 fusion protein expressed by promoter BBa_J23110 that is included in BBa_K4060503.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 910
    Illegal SpeI site found at 946
    Illegal PstI site found at 553
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 910
    Illegal NheI site found at 144
    Illegal NheI site found at 167
    Illegal SpeI site found at 946
    Illegal PstI site found at 553
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 910
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 910
    Illegal SpeI site found at 946
    Illegal PstI site found at 553
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 910
    Illegal SpeI site found at 946
    Illegal PstI site found at 553
    Illegal NgoMIV site found at 1102
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None