Coding

Part:BBa_K4060507

Designed by: Cheng-Ju Lu   Group: iGEM21_NYCU-Taipei   (2021-10-21)


BphP1_mCherry fusion protein

Fusion protein of BphP1 and mCherry with a flexible linker between them.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2194
    Illegal SpeI site found at 2230
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal PstI site found at 2587
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2194
    Illegal SpeI site found at 2230
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal PstI site found at 2587
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2194
    Illegal BamHI site found at 416
    Illegal XhoI site found at 2166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2194
    Illegal SpeI site found at 2230
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal PstI site found at 2587
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2194
    Illegal SpeI site found at 2230
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal PstI site found at 2587
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 367
    Illegal NgoMIV site found at 402
    Illegal NgoMIV site found at 1650
    Illegal NgoMIV site found at 2152
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1207
    Illegal BsaI.rc site found at 2053


[edit]
Categories
Parameters
None