pJ113 is one of our tandem promoter design
K206000 + J23116
These tandem promoters are composed of the pBad(BBa_K206000) and a constitutive promoter with the RFP gene on the downstream. We digested the vector fragment BBa_K206000 with PstI and SpeI and the insert fragment constitutive promoter with RFP by XbaI and PstI. We ligated these fragments together to form pJ102, pJ118, pJ110, pJ116, and pJ113.
Agarose electrophoresis in order to verify the tandem promoters. Lane 1, 100bp ladder. Lane 2, pJ116 with BamHI and PstI. Lane 3, pJ118 with BamHI and PstI. Lane 4, pJ113 with BamHI and PstI. Lane 5, pJ110 with BamHI and PstI. Lane 6, pJ102 with BamHI and PstI.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 125
Illegal NheI site found at 145
Illegal NheI site found at 168
- 21Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC