Part:BBa_K4060110
Const1
Since bioparts may behave differently in different host strains, any team who wish to integrate this kill switch system into their designs can use Construct 2 (BBa_K4060111) to roughly estimate MazE expression level in various condition. After that, a constitutive promoter with appropriate strength could be selected to construct a MazF constant expression system. Strength of constitutive promoters can be test out with an RFP reporter system, and we found that BBa_J23100 constitutive promoter family on BBa_J61002 reporter plasmid is useful for this purpose. A promoter that shows a slightly higher level of RFP expression in comparison with that of Construct 2 (BBa_K4060111) under L-arabinose induction (1mM) or low temperature (20℃) should be chosen. Integrate Construct 1 (BBa_K4060110) with the aforementioned MazF constant expression system in the same plasmid, and the kill switch system is completed.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1112
Illegal XbaI site found at 1128
Illegal SpeI site found at 1382 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1112
Illegal NheI site found at 125
Illegal SpeI site found at 1382
Illegal NotI site found at 1119 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1112
Illegal BamHI site found at 65
Illegal BamHI site found at 1179 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1112
Illegal XbaI site found at 1128
Illegal SpeI site found at 1382 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1112
Illegal XbaI site found at 1128
Illegal SpeI site found at 1382 - 1000COMPATIBLE WITH RFC[1000]
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