BBa_J23106 + BBa_K4060106
The gene fragment Pre C2 is composed of BBa_J23106 and the Pre B3. We digested the vector fragment Pre B3 with EcoRI and XbaI and the insert fragment BBa_J23106 with EcoRI and SpeI. We ligated these fragments together to form Pre C2.
Agarose electrophoresis in order to verify Pre C2. Lane 1, 100bp ladder. Lane 2,3, Pre C2 digested with NheI.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 168
- 21Illegal BamHI site found at 108
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 754
Illegal AgeI site found at 866
- 1000COMPATIBLE WITH RFC