Plasmid
CRISPRa

Part:BBa_K4058010

Designed by: Sarah Cumberland   Group: iGEM21_Guelph   (2021-10-16)


pHSN6A01 (CRISPRa plasmid)

pHSN6A01 is a CRISPR activation (CRISPRa) plasmid. It codes for a fusion protein containing dCas9 fused to a VP64 transcriptional activation domain. This fusion protein binds to a specific region of DNA based on the associated gRNA, but it does not cleave the DNA like Cas9. Instead, the VP64 domain recruits transcription factors to its location and thereby increases transcription from the targeted gene. In pHSN6A01, dCas9-VP64 is under constitutive control from the promoter CaMV35s. No guide RNA is present in pHSN6A01.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7833
    Illegal XbaI site found at 2961
    Illegal SpeI site found at 9996
    Illegal PstI site found at 2032
    Illegal PstI site found at 3326
    Illegal PstI site found at 3872
    Illegal PstI site found at 5294
    Illegal PstI site found at 5498
    Illegal PstI site found at 6740
    Illegal PstI site found at 12904
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7833
    Illegal NheI site found at 4538
    Illegal SpeI site found at 9996
    Illegal PstI site found at 2032
    Illegal PstI site found at 3326
    Illegal PstI site found at 3872
    Illegal PstI site found at 5294
    Illegal PstI site found at 5498
    Illegal PstI site found at 6740
    Illegal PstI site found at 12904
    Illegal NotI site found at 2953
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7833
    Illegal BglII site found at 4128
    Illegal BglII site found at 4818
    Illegal BamHI site found at 2930
    Illegal BamHI site found at 4422
    Illegal BamHI site found at 6460
    Illegal BamHI site found at 7557
    Illegal XhoI site found at 2922
    Illegal XhoI site found at 3746
    Illegal XhoI site found at 4928
    Illegal XhoI site found at 6092
    Illegal XhoI site found at 8657
    Illegal XhoI site found at 9751
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7833
    Illegal XbaI site found at 2961
    Illegal SpeI site found at 9996
    Illegal PstI site found at 2032
    Illegal PstI site found at 3326
    Illegal PstI site found at 3872
    Illegal PstI site found at 5294
    Illegal PstI site found at 5498
    Illegal PstI site found at 6740
    Illegal PstI site found at 12904
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7833
    Illegal XbaI site found at 2961
    Illegal SpeI site found at 9996
    Illegal PstI site found at 2032
    Illegal PstI site found at 3326
    Illegal PstI site found at 3872
    Illegal PstI site found at 5294
    Illegal PstI site found at 5498
    Illegal PstI site found at 6740
    Illegal PstI site found at 12904
    Illegal NgoMIV site found at 1457
    Illegal NgoMIV site found at 4690
    Illegal NgoMIV site found at 10733
    Illegal AgeI site found at 9030
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1746
    Illegal BsaI.rc site found at 533
    Illegal SapI site found at 3664
    Illegal SapI.rc site found at 5131


[edit]
Categories
Parameters
None