Plasmid_Backbone

Part:BBa_K4047018

Designed by: An Nguyen   Group: iGEM21_MiamiU_OH   (2021-10-04)


dSBPase-Backbone

Backbone sequence used for PCR assembly of our SBPase-deletion plasmid. This sequence contains the promoter, coding portion, and terminator for gentamicin (GmR). It also contains an origin of replication for the plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 1489
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 864


[edit]
Categories
//plasmidbackbone
Parameters
None