DNA
MHETase

Part:BBa_K4043002

Designed by: Jie Wang   Group: iGEM21_United_Shanghai_HS   (2021-09-22)


MHETase expressed genes in frame

MHETsae was also found in this kind of bacteria, and it was confirmed that it can be expressed in conjunction with PETase to further decompose. MHETase can further decompose mhetase, the main product metabolized by PETase, and degrade it into TPA and EG. TPA and EG can enter the bacteria's own metabolic pathway for complete degradation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 648
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 648
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1331
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 648
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 648
    Illegal NgoMIV site found at 34
    Illegal NgoMIV site found at 330
    Illegal NgoMIV site found at 748
    Illegal NgoMIV site found at 786
    Illegal AgeI site found at 283
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None