DNA
PETase

Part:BBa_K4043001

Designed by: Jie Wang   Group: iGEM21_United_Shanghai_HS   (2021-09-22)


In-frame expression genes of PETase

ISF6_4831 has a high sequence similarity with the PET hydrolyzing horn, but its hydrolytic activity and selectivity for PET are significantly higher than other PET hydrolyzing enzymes. In addition, we found in the literature that the Wu Bian team of the Institute of Microbiology of the Chinese Academy of Sciences optimized the enzyme ISF6_4831 required by the Osaka bacteria to degrade PET plastics, and obtained a redesigned enzyme with significantly enhanced stability, which made the Osaka bacteria to PET film The degradation efficiency is increased by 300 times. We used its optimized ISF6_4831 protein sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 166
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 141


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Categories
Parameters
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