Coding

Part:BBa_K4040011

Designed by: Tong Yin   Group: iGEM21_NMU_China   (2021-09-14)


tTA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

tTA(tetR-VP64) contains three core domains from N-terminal to C-terminal: DNA-binding transcriptional repressor(TetR), nuclear location sequence and VP64 transcription regulating domain. And a linker was added between DBD and NLS for providing region flexibility. tetRDBD enable binding to specific DNA sequences, so that we can use tetR-VP64 as a specific transcription factor to activate the expression of our downstream synthetic promoter elements and CMV. The usage and experimental results in our project can be found in IL-6R-TCS-tTA(BBa_K4040022).



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