DNA

Part:BBa_K4029003

Designed by: JUAN LIANG-HSUEH   Group: iGEM21_CSMU_Taiwan   (2021-10-14)


miR-664a-3p binding site on Circular DNA

miR-664a-3p binding site on Circular DNA

Design

Using the RNA sequence of miR-664a-3p(refer to miR Base[1]), we designed BBa_K4029003 using complementary base pairing with miR-664a-3p to bind it to our Circular DNA.

Function

BBa_K4029003 acts as a binding site to miR-664a-3p, to capture our target microRNA used in our project. BBa_K4029003 allows miR-664a-3p to act as a primer to start the RCA reaction in the presence of phi29 polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None