DNA
Part:BBa_K4029003
Designed by: JUAN LIANG-HSUEH Group: iGEM21_CSMU_Taiwan (2021-10-14)
miR-664a-3p binding site on Circular DNA
miR-664a-3p binding site on Circular DNA
Design
Using the RNA sequence of miR-664a-3p(refer to miR Base[1]), we designed BBa_K4029003 using complementary base pairing with miR-664a-3p to bind it to our Circular DNA.
Function
BBa_K4029003 acts as a binding site to miR-664a-3p, to capture our target microRNA used in our project. BBa_K4029003 allows miR-664a-3p to act as a primer to start the RCA reaction in the presence of phi29 polymerase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |