DNA

Part:BBa_K4029001

Designed by: JUAN LIANG-HSUEH   Group: iGEM21_CSMU_Taiwan   (2021-10-13)


Circular DNA Binding Site on Immobilisation Probe

Design

BBa_4029001 is our Circular DNA Binding Site on Immobilisation Probe. This basic part allows the Immobilisation Probe used in our mechanism GotCha to bind to the Circular DNA through complementary base pairing. With BBa_4029001, it is possible to anchor our circular DNA to magnetic beads for RCA reaction.

Function

Immobilisation Probe is used in our mechanism GotCha to bind to the Circular DNA through complementary base pairing. With BBa_4029001 and BBa_K4029002, it is possible to anchor our circular DNA to magnetic beads for RCA reaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None