Part:BBa_K4025000

Pchb is a promoter codon-optimized for E. coli that originates from Vibrio cholerae’s chitin utilization program. This promoter has two ChiS binding sites and a SlmA binding site for activation– SlmA originating from both E. coli and V. cholerae’s genome. We coupled Pchb with sfGFP to report activation induced by chitin.

Pchb coupled with an sfGFP reporter was transformed into plasmid backbone pSB1k04 and a growth curve was constructed to measure cell burden. See: https://2021.igem.org/Team:Lubbock_TTU/Contribution.

As can be seen from the growth curves, the L1 transcriptional unit with Pchb did not overly burdened cell growth– all transformed cells survived. It should be noted; however, that the OD600 readings of all cultures were not standardized prior to inoculation, seen by the varying y-intercepts of all growth curves. Because of this, this data should not be used as quantitative measurements of growth kinetics, but rather a qualitative demonstration of relative growth.