Composite

Part:BBa_K4019011

Designed by: Ozora Ishikawa   Group: iGEM21_TokyoTech   (2021-10-20)


Bacteriocin biosynthesis gene cluster

This part is for biosynthesis of bacteriocin which is originally produced by Lactiplantibacillus pentosus H99. L.pentosus H99 is known to live in the intestines of cattle and produce bacteriocin which has antimicrobial activity. Its antimicrobial activity against Streptococcus mutans is confirmed in our project. The gene cluster contains 4 sets of genes: Bacteriocin precursor, Bacteriocin immunity protein, PedC/BrcD family Bacteriocin maturation disulfide and ABC transporter ATP-binding protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2645
    Illegal SpeI site found at 1835
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 185
    Illegal SpeI site found at 1835
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2645
    Illegal SpeI site found at 1835
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2645
    Illegal SpeI site found at 1835
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None