Coding

Part:BBa_K4019002

Designed by: Ozora Ishikawa   Group: iGEM21_TokyoTech   (2021-10-17)


Optimized ScCCR (4-coumarate-CoA ligase from Streptomyces coelicolor)

To biosynthesize cinnamaldehyde from L-phenylalanine takes three steps, and it required three enzymes, phenylalanine-ammonia lyase, 4-coumarate-CoA ligase, cinnamoyl-CoA reductase. This part, Optimized ScCCL, catalyzed acid-thiol ligation of cinnamic acid to cinnamoyl- CoA. This part is optimized for L.lactis subsp. cremoris by IDT, Inc. Codon Optimization Tool.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 506
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 260
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 506
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 178


[edit]
Categories
Parameters
None