Part:BBa_K4016007
VK_cyclinE1
VK_cyclinE1 module is designed to interact with VH_cyclinE1 and form a folded conformation, which can recognize cyclinE1 and generate its degradation.
Usage and Biology
Hybridoma cell line HE12 and HE172 can produce anti-cyclinE proteins, but they have different biochemistry properties. Each has its own merits and drawbacks. Therefore, with the goal of generating a well-performed anti-cyclinE protein, the DNA sequences from those two cell lines were rearranged, and by RT-PCR a new anti-cyclinE protein was generated. It was reported as a kind of intrabodies, namely, anti-cyclinE single-chain antibodies (ScFv). VK_cyclinE1 comes from hybridoma-rearranged light chain variable region gene in HE12 and HE172 hybridomas. By the same way, VH_cyclinE1 is produced from heavy chain variable region gene. We assumes that VH and VK will take on a folded conformation when they are adjacent in three-dimensional space, and thus recognize and bind to CyclinE tightly and specifically.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
[1] Randall W. Strube , Si-Yi Chen, et al. Characterization of anti-cyclin E single-chain Fv antibodies and intrabodies in breast cancer cells: enhanced intracellular stability of novel sFv–Fc intrabodies[J].Journal of Immunological Methods, 2002, 263: 149–167.
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