Coding

Part:BBa_K4010004

Designed by: iGEM_McMaster   Group: iGEM21_McMaster   (2021-10-10)


TorA-Linked Colicin E1 and Immunity Protein

The colicin E1 with a his-tag (BBa_K4010001) is tagged with a TorA signal sequence (BBa_K3114005) at the N-terminal. The colicin is cleaved from the E1 immunity protein (BBa_K4010002) by a 2A-like linker (BBa_K2976010). The E1 immunity protein embeds into the inner membrane. The TorA signal sequence permits the migration of E1 colicin through the inner membrane using the native Tat transportation system. Secretion into the extracellular space is carried out by the Type 2 secretion system. The his-tag allows purification and quantification purposes of colicin E1.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1833
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1833
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1833
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1833
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 133
    Illegal SapI site found at 439


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