Coding

Part:BBa_K4010001

Designed by: iGEM_McMaster   Group: iGEM21_McMaster   (2021-10-15)


Isolated Colicin E1 with His-Tag

Colicin E1 is a bacteriocin produced by certain E. coli to kill closely related strains via btuB receptor binding to form channels that depolarizes bacterial inner membranes. The His-tag is added for purification purposes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 301


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Categories
Parameters
None