Composite
Part:BBa_K4007004
Designed by: Jonatan Joel Aliaga Rodriguez Group: iGEM21_Bolivia (2021-10-06)
Ultrasensitive biosensing complex por arsenic (#1)
Biosensing complex por arsenic that comprises of a weak constitutive promoter that is in charge of the expression of the arsR protein. Next is the processing module that is made up of the Pars inducible promoter, which receives the transcriptional signal, and a double amplifying cascade (HrpRS-pHrpL-RinA). Finally, the output module is made up by an inducible promoter pRinA-p80 that receives the amplified signal of the cascade and promotes the expression of the reporter gene mRFP1-Violet. The use of a double amplifying cascade makes its reponse ultrasensitive to arsenic with sub-5 ppb sensitivities.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 198
Illegal BglII site found at 2442 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3962
Illegal AgeI site found at 4074 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1652
Illegal SapI.rc site found at 2285
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Categories
Parameters
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