Reporter

Part:BBa_K4007000

Designed by: Jonatan Joel Aliaga Rodriguez   Group: iGEM21_Bolivia   (2021-10-05)


mRFP1 violet variant

Violet chromoprotein that has a low molecular weight (55 kDa), it has been reported that it's toxicity to E. coli is considerably low relative to other chromoproteins, which is a great advantage for its use as a reporter. Its maturation time is 18 - 24 hours in the presence of oxygen. Has a fluorescent capability with an excitation peak at 586 nm and an emission peak at 619 nm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/chromoprotein
Parameters
None